2, 4-Dinitrophenylhydrazine extra pure

2,4-Dinitrophenylhydrazine extra pure (DNPH) is a high-purity laboratory-grade chemical widely used as a qualitative reagent in organic chemistry, particularly for the identification of aldehydes and ketones (carbonyl compounds).​

Chemical Properties

Chemical name: 2,4-Dinitrophenylhydrazine (DNPH, Brady’s reagent)

Molecular formula:

C

6

H

6

N

4

O

4

C

6

H

6

N

4

O

4

​

Molecular weight: 198.14 g/mol​

CAS Number: 119-26-6​

Appearance: Orange-red to dark red crystalline powder, sometimes moist​

Purity (Assay): Minimum 99% (extra pure/reagent grade)​

Melting point: 198–202°C​

Solubility and Handling

Solubility: Slightly soluble in water, soluble in alcohol, ether, and 50% sulfuric acid.​

Shelf life: Typically 5 years (60 months) if stored at room temperature (25–40°C) in a tightly sealed container.​

Safety:

Hazardous if inhaled, ingested or if it comes in contact with skin/eyes.​

Shock-sensitive in dry form; often shipped and handled moistened with water.​

Use standard laboratory PPE; avoid sources of ignition and static discharge.​

Major Uses

DNPH Test (Brady’s Test): Primary reagent for detecting and characterizing aldehydes and ketones by formation of brightly colored hydrazone derivatives.​

Environmental Chemistry: Used for monitoring air pollutants—especially carbonyl compounds like formaldehyde—in occupational and environmental settings.​

Chromatographic Identification: Hydrazone derivatives produced are useful for TLC, HPLC, and melting point analysis aiding compound identification.​

Structural Elucidation: Used in research for elucidating structures of various organic compounds.​