2,6-Dichlorophenol Indophenol"

2,6-Dichlorophenol Indophenol (often referred to as DCPIP or DCIP) is a synthetic, water-soluble redox indicator and dye, widely used in analytical, biochemical, and food chemistry.​

Chemical Properties

Chemical name: 2,6-Dichlorophenolindophenol (sodium salt often used in labs)

Molecular formula (sodium salt):

C

12

H

6

Cl

2

NNaO

2

C

12

H

6

Cl

2

NNaO

2

​

Molecular weight (sodium salt): 290.08 g/mol​

CAS Number: 620-45-1 (sodium salt)​

Appearance: Dark blue to dark green powder or crystals; dark green (sodium salt dihydrate)​

Solubility: Water (30 g/L), alcohol (20 g/L)​

Purity (AR/ACS): 98–106% (commercial, for analysis grade)​

Major Uses

Redox Indicator: Exhibits a blue color when oxidized and turns colorless (or pale yellow) when reduced.​

Vitamin C (Ascorbic Acid) Analysis: Commonly used in titration methods for quantitative estimation of ascorbic acid in food, juice, and pharmaceuticals. When ascorbic acid reduces DCPIP, the solution becomes colorless, marking the endpoint.​

Respiratory/Photosynthetic Studies: Serves as an artificial electron acceptor in experiments studying the electron transport chain in plants (photosynthesis) and mitochondrial activity.​

Biochemical Assays: Used as a substrate to measure enzyme activities, including dehydrogenases and oxidases, and as a reagent in TLC for organic acids and reducing compounds.​

Additional Features

pKa: 10.5 (indicative of its behavior as an acid/base)​

Shelf life: Typically up to 5 years​

Storage: Store at room temperature, protected from light and moisture (the dye is hygroscopic)​

Safety

Hazard statements: May cause eye/skin irritation; standard PPE (gloves, eye protection) is recommended.