Carbol Fuchsin Powder For Microscopy
Description
Carbol fuchsin powder for microscopy is a dry, certified-grade mixture of basic fuchsin dye used to prepare staining solutions for acid-fast microscopy, particularly Ziehl-Neelsen and Kinyoun methods.
Composition
The powder primarily consists of basic fuchsin (pararosaniline hydrochloride, C.I. 42500), a triarylmethane dye with high purity (typically 85–100% dye content) suitable for reproducible staining; it is dissolved in phenol, alcohol, and water to make the final carbol fuchsin solution.
Preparation and use
To prepare strong Ziehl-Neelsen solution, about 1–10 g of the powder (adjusted for dye content) is dissolved in ethanol or methanol, then mixed with 5% aqueous phenol; the resulting dark red solution is filtered for clarity.
It stains acid-fast bacteria (e.g., Mycobacterium spp.) bright red by penetrating waxy cell walls when heated, resisting decolorization by acid-alcohol.
Quality standards
Microscopy-grade powder meets Biological Stain Commission certification, ensuring low impurities, consistent color intensity (e.g., extinction coefficient), and no precipitates that could interfere with staining.
Specifications
| Model No. | N-CH1537 |
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