EDTA Disodium Salt 5% Solution

EDTA Disodium Salt 5% Solution is an aqueous preparation containing 5 grams of EDTA disodium salt per 100 milliliters of water. It is commonly used as a chelating agent in clinical, research, and industrial applications to sequester divalent and trivalent metal ions.​

Properties

Composition: 5% w/v (5 g per 100 ml water).

Appearance: Clear, colorless solution; soluble in water; derived from a white crystalline solid.​

pH of 5% Solution: 4.0 to 6.0 (acidic).​

Chelation Value: 260–290 mg CaCO₃ per gram (typical for high-purity disodium EDTA salt).​

Common Uses

Hematology: Used as an anticoagulant in blood collection tubes to prevent clotting by binding calcium.​

Laboratory: Chelates metal ions in buffers, enzyme preparations, and nucleic acid isolation protocols.

Industrial: Added to water systems or cleaning solutions to bind calcium, magnesium, iron, and other metals.​

Conservation: Removes rust and stains from stone, wood, and fresco surfaces by complexing metallic ions.​

5% EDTA Disodium Salt Solution is valued for its strong metal-binding ability and is used in numerous fields for sample stabilization, protein and DNA preservation, and removal of unwanted metallic contaminants.