Evans blue

Evans Blue is an azo dye with the molecular formula C34H24N6Na4O14S4 and a molecular weight of approximately 960.8 g/mol. It appears as a dark brown to black powder and forms a blue solution when dissolved in water. Evans Blue dye has high water solubility (around 280 g/L at 20 ºC) and is known for its strong affinity to serum albumin, making it a widely used tracer for blood volume and vascular permeability studies.

Key Properties:

Chemical formula: C34H24N6Na4O14S4

Appearance: Dark brown to black powder

Solubility: Highly soluble in water, slightly soluble in ethanol

Absorption maxima: Around 606-611 nm

Dye content: Typically ≥85%

Color: Blue in solution

Applications:

Used as a biological stain and vascular tracer in biochemical and physiological research

Employed in microscopy and histology as a dye to identify damaged cells and to stain connective tissues

Utilized in medical diagnostics to measure blood volume and vascular integrity

Acts as a fluorochrome in fluorescence microscopy for background autofluorescence suppression

Safety and Handling:

Generally safe in laboratory abundances but should be handled with care to avoid skin or eye contact

Store in a cool, dry place protected from light

This comprehensive profile is suitable for product catalogs, research reagent datasheets, and biochemical supply platforms.​